HbAc test comparison -
Updated August 31, Horowitz GL, Ucciferro, P, Anastasopoulou, C. Hemoglobin A1c Testing. Devaraj S, ed. Updated October 4, Inzucchi SE, Lupsa B.
Clinical Presentation, Diagnosis, and Initial Evaluation of Diabetes Mellitus in Adults. In: Nathan DM, Wolfsdorf JI, eds. Updated October 29, MedlinePlus: National Library of Medicine.
Hemoglobin A1C HbA1c Test. Updated September 15, National Heart, Lung, and Blood Institute. Blood Tests. Updated March 24, National Institute of Diabetes and Digestive and Kidney Diseases. Updated December Updated April Insulin Resistance and Prediabetes.
Updated May Updated August Nielsen LR, Ekbom P, Damm P, et al. HbA1c Levels are Significantly Lower in Early and Late Pregnancy. Diabetes Care. Selvin E. Measurements of Glycemic Control in Diabetes Mellitus.
Updated February 15, Whitley HP, Yong EV, Rasinen C. Selecting an A1C Point-of-Care Instrument. Diabetes Spectr. This form enables patients to ask specific questions about lab tests. Your questions will be answered by a laboratory scientist as part of a voluntary service provided by one of our partners, American Society for Clinical Laboratory Science.
Please allow business days for an email response from one of the volunteers on the Consumer Information Response Team. Last modified on Sep 13, Hemoglobin A1C HbA1c Test Also Known As: A1C Test, Glycated Hemoglobin Test, HbA1C, Hgba1c, Hgb a1c.
Board Approved. Medically Reviewed by Thomas Kampfrath, PhD, DABCC, FAACC. Get tested. Test Quick Guide The hemoglobin A1c test estimates how much glucose, or blood sugar, has been in your bloodstream over the last three months.
About the Test Purpose of the test The hemoglobin A1c test may have several purposes: Screening for prediabetes and diabetes: If you are over 40 and are obese, overweight, or otherwise at high risk for diabetes, your doctor may want to check for health conditions before you experience symptoms.
The test can be used for diabetes screening, but the results may need to be confirmed by repeating the test or using another type of test. Diagnosing prediabetes and diabetes: This uses tests and procedures to find out the cause of health changes. Your doctor may order hemoglobin A1c and other tests if you have symptoms or show signs of diabetes.
Symptoms can include excessive thirst, frequent urination, blurred vision, tingling or loss of sensation in the feet and hands, and feeling extremely tired. Monitoring diabetes: If you have been diagnosed with diabetes, your doctor may order this test at least twice a year.
This gives your doctor an idea of how well your blood glucose has been controlled in the months between appointments and allows your doctor to make adjustments to your treatment plan and lower your chance of health problems. What does the test measure?
When should I get this test? htm A. htm American Diabetes Association. html Centers for Disease Control and Prevention. html Hayward RA, Selvin E. See More.
A1c Gear: Laboratory quality HbA1c measurementat the point of care. Three of 7 hemoglobin A1c point-of-careinstruments do not meet generally accepted analytical performance criteria. Strojek K, Potyralska MM. Ocena przydatności oznaczania stężenia HbA1c dodiagnostyki cukrzycy w wybranej próbie populacji polskiej.
Malkani S, Korpi-Steiner N, Rao LV. Reducing analytical variation between point-of-care and laboratory HbA1c testing. J Diabetes. Sánchez-Mora C, S Rodríguez-Oliva M, Fernández-Riejos P, et al. Evaluationof two HbA1c point-of-care analyzers. Wan Mohd Zin RM, Ahmad Kamil ZI, Tuan Soh TR, et al.
Haemoglobin A1c:comparing performance of two point of care devices with laboratory analyser. BMC Res Notes. Shephard MDS, Gill JP.
Clin Biochem Rev. Goodall I, Colman PG, Schneider HG, et al. Desirable performance standards forHbA 1c analysis — precision, accuracy and standardisation: consensus statementof the Australasian Association of Clinical Biochemists AACB , the AustralianDiabetes Society ADS , the Royal College of Pathologists of Australasia RCPA ,Endocrine Society of Australia ESA , and the Australian Diabetes EducatorsAssociation ADEA.
Westgard QC. A War of Words in Laboratory Medicine, part IV: The Quality ofGlycated Hemoglobin Ghb. Dostęp: Evaluation of the Quo-Test hemoglobin A1cpoint-of-care instrument: second chance.
Hemoglobin A1c point-of-care assays; a newworld with a lot of consequences! J Diabetes Sci Technol. Solnica B. Correlation coefficient for Micromat II and immuno-turbidimetric analysis was 0.
Medical staff found the same-visit measurement difficult to perform due to the amount of dedicated time required for the test. For each of the laboratory methods, the correlation coefficient was lower than the 0. This might be due to variability introduced by the multiple users of the Micromat II machine.
The mean HbA1c results were also consistently lower than those obtained from laboratory analysis. Additionally, the amount of dedicated time required to perform the assay may limit its usefulness in a busy clinical practice.
Before introducing a same-visit HbA1c methodology, clinicians should compare the rapid results to their current method of analysis. Peer Review reports. The percent HbA1c of glycated hemoglobin provides an estimate of blood glucose levels over a 3—4 month period.
The HbA1c level is used for patient education and counseling, for feedback about diabetic control, to improve patient motivation, and to monitor management; thus its measurement should be optimally accurate and precise [ 1 ].
However, to date, there is no international standard for determining HbA1c [ 2 — 4 ], and various methodologies are commercially available. Tran [ 1 ] determined the physiological changes over time between measurements and analytic variation of two widely used laboratory assays, one a high performance liquid chromatography HPLC method, and the other an immunoassay [ 1 ].
The coefficient of variation CV for the HPLC was 2. The 5. Hosseini et al. They found that glycated hemoglobin results vary widely, with some assays consistently more likely to result in a "normal glycated hemoglobin" level than other assays, consequently resulting in differing implications for an individual patient to achieve a HbA1c level within the normal range.
Ogawa [ 6 ] reported a case series where HbA1c was underestimated in the measurement by HPLC which excluded glycated abnormal hemoglobin [ 6 ]. These findings illustrate the potential usefulness for clinical practitioners to evaluate the performance of their method for determining HbA1c, especially if using different methodologies for the same patient.
Recent developments in medical technology allow clinicians to determine HbA1c test results during a patient's office visit. Several manufacturers offer an assay that can be performed by trained medical personnel and yield HbA1c results in five to ten minutes.
We found only a few reports of the performance of such rapid tests used at the point of care [ 7 , 8 ], and one study that was conducted by the test manufacturer [ 9 ]. The objective of this pilot study was to test the feasibility of introducing a same-visit HbA1c methodology into busy family practice centers FPCs and to compare the results obtained from a point-of-care test with a laboratory-based technique.
Specifically, our purpose was to determine: 1 if a specific rapid HbA1c methodology was accepted by medical support staff in two busy FPCs; and 2 how rapid HbA1c results compared with the standard laboratory methodology.
Patients were recruited for this cross-sectional study from two FPCs that are members of MetroNet, a metropolitan Detroit practice-based research network. At both sites, HbA1c analysis is routinely performed at an outside laboratory on venipuncture samples.
Physicians, medical assistants, and research assistants identified consecutive diabetic patients 18 years of age and older whose physicians ordered HbA1c analysis.
The study was explained to these eligible patients and informed consent obtained from those who wished to participate. After patients were enrolled, a finger-prick blood sample was collected for in-office HbA1c testing with the BIO-RAD Micromat II.
Since the BIO-RAD Micromat II is compatible with capillary, venous, and EDTA anti-coagulated blood samples, aliquots of these types were also acceptable for analysis. Research and medical staff were instructed to use finger-prick capillary samples whenever possible, but venous samples from the blood draw apparatus, or a drop of blood from the EDTA tube was substituted when necessary.
At one FPC only finger-prick samples were used, while at the other FPC, thirteen MicroMat II samples were venous and five were EDTA anti-coagulated; the remaining 56 tests were performed using capillary blood samples.
The data collected included patient name, study site, the person performing same-visit HbA1c analysis, the date, and the rapid HbA1c result.
Physicians were blinded to rapid HbA1c results, and relied on the laboratory analysis to make treatment decisions during the study period. One FPC used one of two different laboratories based on the patient's health insurance carrier.
At one laboratory, the Primus Model was used for HbA1c testing, which is a boronate — affinity HPLC method. The other laboratory used the Roche Integra , which uses an immuno-turbidimetric methodology. The laboratory of the second FPC used the Tosoh A1c 2.
All three methodologies are aligned to Diabetes Control and Complications Trial DCCT and National Glycohemoglobin Standardization Program NGSP standards. The intra- and inter-assay coefficients of variation are displayed in Table 1. These values were either obtained directly from the laboratory performing the assay Primus or from the manufacturer.
All are within NGSP acceptable limits. The BIO-RAD Micromat II, which provides results in approximately 5 minutes, incorporates an affinity chromatography method that measures the percent glycated hemoglobin in the sample.
According to the manufacturer, the analyzer then uses a factory-set algorithm to deliver an HbA1c result which is calibrated to the recommendations of the DCCT and is traceable to the NGSP. The intra-assay coefficient of variation is reported to range from 2.
The inter-assay coefficient of variation is estimated to be higher; however values are not given in the package insert.
BIO-RAD representatives provided an in-service to help familiarize staff in the use and operation of the Micromat II analyzer. Each HbA1c analysis with the Micromat II requires a single test cartridge, which consists of several tubes with reagents that are mixed and decanted into a collection reservoir for measurement.
After a test cartridge has been placed into the Micromat II, a 20 microliter blood sample is added to the first tube. This initiates a series of aliquot additions and incubation steps. In total there are four decanting steps followed by four incubations.
These incubations require a total time of seconds and range from 40 seconds to 80 seconds in length. Quality control procedures were carried out as outlined in the Micromat II instruction manual. Controls and standards were run per the manufacturer's recommendation; results were always acceptable.
Data were analyzed separately by type of laboratory methodology. To evaluate the performance of the BIO-RAD Micromat II, Pearson correlations were calculated using the laboratory results as the standard.
Scatter plots and regression lines were also examined. One hundred fifty-six patients were enrolled into the study 75 from one FPC, and 81 from the other FPC.
Nine different medical staff performed the rapid HbA1c testing. Therefore, paired samples were available for analysis, 73 from one site and 74 from the other. The range of values was from 2. The laboratory method yielded a mean HbA1c value that was significantly higher than that from the Micromat II for both methodologies 7.
Thank you for visiting nature. You are using a tesg version with limited comparsion for HbAAc. To obtain the best experience, we recommend you use a more up to date HhAc Herbal remedies for urinary tract infections turn off Herbal remedies for urinary tract infections mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. The prevalence of diabetes in Singapore is high. Screening to facilitate early detection and intervention has been shown to be cost-effective. Current clinical practice guidelines in Singapore recommend screening with fasting plasma glucose FPGfollowed by an oral glucose tolerance test OGTT in those with FPG 6. Glycated comparisoh, or HbA1c, is Compparison main biomarker Nutrition periodization for performance to assess compparison glycaemic control in individuals with diabetes, and it Athletic performance strategies with the compxrison of complications. Compagison aim of this article is to provide an overview of HbA1c bHAc understand its role in the treatment of individuals living with diabetes. Topics discussed include recommended treatment targets, methods of measurement, causes of measurement inaccuracy and alternative means available to assess glycaemic control. HbA1c should not be interpreted in isolation; the measurement accuracy and other parameters, including treatment goals and comorbidities, need to be considered. The use of glycated haemoglobin, or HbA1c, has become the standard of assessing glycaemic control in patients with diabetes since the American Diabetes Association ADA recommended its use in
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