Free access. Glycogen synthase kinase-3 GSK-3 is Clean eating snacks synthsis kinase that regulates signalling pathways affecting glycogen metabolism, protein synthesis, mitosis, and apoptosis. Mora, A.

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Glycogen metabolism

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Thus, in human muscle cells, part of the insulin-sensitising effect of rosiglitazone can be attributed to changes in mRNA and protein expression of glucose transporters. AICAR activates AMPK and stimulates glucose uptake in skeletal muscle [ 45 ]. In the present study, acute AICAR treatment increased glycogen synthesis, while chronic 8 days AICAR treatment was without effect on insulin action, as assessed by glycogen synthesis, or expression of GLUT4 or PGC1 mRNAs.

The acute responses are consistent with findings in rat skeletal muscle [ 31 , 46 ] and perfused hindlimb [ 47 ], where AICAR treatment increased GLUT4 expression and translocation respectively. Furthermore, chronic AICAR treatment of Clone 9 cells increased glucose uptake [ 48 ].

Based on the observation that GLUT1 content was unchanged in the plasma membranes of AICAR-treated Clone 9 cells, the effects on glucose uptake were proposed to activate pre-existing plasma membrane GLUT1 transporters. Nevertheless, our present data reveal that chronic AICAR treatment in human myotubes selectively increases GLUT1, but not GLUT4, expression.

Moreover, in human myotubes exposed to AICAR for 8 days, mRNA expression of MEF2C was reduced. Whether AICAR has a negative effect on the regulation of myogenesis in this cell system remains to be demonstrated, but no such effects have previously been suggested. However, MEF2C is involved in the regulation of differentiation-specific genes [ 16 ] and suppression of this gene may repress gene-regulatory responses.

Metformin treatment of cells increases GLUT4 recruitment and increases glycogen synthesis [ 49 ]. Here we demonstrate that chronic metformin exposure increases the expression of GLUT4, PGC1 and all MEF2 isoforms A, C and D and increases the rate of basal and insulin-stimulated glycogen synthesis.

Metformin has been suggested to increase AMPK activity [ 29 ]. Interestingly, in human myotubes, metformin treatment was more potent than AICAR in increasing GLUT4 expression and in enhancing insulin sensitivity, suggesting that metformin activates additional AMPK-independent pathways.

In summary, exposure of human myotubes to metformin and rosiglitazone, two currently available antidiabetic agents, was associated with direct enhancement of the action of insulin in cultured skeletal muscle. The increase in insulin action correlated with increased mRNA expression of GLUT4.

Increased expression of PGC1, GLUT4 and MEF2 mRNA after antidiabetic treatment provides a molecular mechanism for increased carbohydrate metabolism, cellular survival and gene-regulatory responses that confer improved skeletal muscle metabolic function in insulin-resistant and diabetic subjects.

McGarry JD Glucose—fatty acid interactions in health and disease. Am J Clin Nutr S—S. CAS PubMed Google Scholar. Boden G Role of fatty acids in the pathogenesis of insulin resistance and NIDDM. Diabetes — Ciaraldi TP, Abrams L, Nikoulina S, Mudaliar S, Henry RR Glucose transport in cultured human skeletal muscle cells.

Regulation by insulin and glucose in nondiabetic and non-insulin-dependent diabetes mellitus subjects. J Clin Invest — Kelley DE, Goodpaster BH Effects of exercise on glucose homeostasis in type 2 diabetes mellitus. Med Sci Sports Exerc S—S Lewis GF, Carpentier A, Adeli K, Giacca A Disordered fat storage and mobilization in the pathogenesis of insulin resistance and type 2 diabetes.

Endocr Rev — Article CAS PubMed Google Scholar. Zierath JR, Krook A, Wallberg-Henriksson H Insulin action and insulin resistance in human skeletal muscle. Diabetologia — Charron MJ, Brosius FC III, Alper SL, Lodish HF A glucose transport protein expressed predominately in insulin-responsive tissues.

Proc Natl Acad Sci U S A — James DE, Strube MM, Mueckler MM Molecular cloning and characterization of an insulin-regulatable glucose transporter.

Nature — Tsao T-S, Stenbit A, Li J et al Muscle-specific transgenic complementation of GLUT4-deficient mice: preferential effects of glucose but not lipid metabolism. Shepherd PR, Gnudi L, Tozzo E et al Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue.

J Biol Chem — Torrance CJ, Devente JE, Jones JP, Dohm GL Effects of thyroid hormone on GLUT4 glucose transporter gene expression and NIDDM in rats. Endocrinology — Mora S, Pessin JE The MEF2A isoform is required for striated muscle-specific expression of the insulin-responsive GLUT4 glucose transporter.

Thai MV, Guruswamy S, Cao KT, Pessin JE, Olson AL Myocyte enhancer factor 2 MEF2 -binding site is required for GLUT4 gene expression in transgenic mice: regulation of MEF2 DNA binding activity in insulin-deficient diabetes. Biochem Biophys Res Commun — McKinsey TA, Zhang CL, Olson EN MEF2: a calcium-dependent regulator of cell division, differentiation and death.

Trends Biochem Sci — Mora S, Yang C, Ryder JW, Boeglin D, Pessin JE The MEF2A and MEF2D isoforms are differentially regulated in muscle and adipose tissue during states of insulin deficiency.

Al-Khalili L, Chibalin AV, Yu M et al MEF2 activation in differentiated primary human skeletal muscle cultures requires coordinated involvement of parallel pathways. Am J Physiol Cell Physiol C—C Handschin C, Rhee J, Lin J, Tarr PT, Spiegelman BM An autoregulatory loop controls peroxisome proliferator-activated receptor γ coactivator 1 α expression in muscle.

Puigserver P, Wu Z, Park WC et al A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell — Patti ME, Butte AJ, Crunkhorn S et al Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1.

Sood V, Colleran K, Burge MR Thiazolidinediones: a comparative review of approved uses. Diabetes Technol Ther — Reasner CA Promising new approaches.

Diabetes Obes Metab 1:S41—S Mauvais-Jarvis F, Andreelli F, Hanaire-Broutin H, Charbonnel B, Girard J Therapeutic perspectives for type 2 diabetes mellitus: molecular and clinical insights.

Diabetes Metab — Nambi V, Hoogwerf RJ, Sprecher DL A truly deadly quartet: obesity, hypertension, hypertriglyceridemia, and hyperinsulinemia.

Cleve Clin J Med — PubMed Google Scholar. Ciaraldi TP, Kolterman OG, Scarlett JA, Kao M, Olefsky JM Role of glucose transport in the postreceptor defect of non-insulin-dependent diabetes mellitus. Garg R, Tripathy D, Dandona P Insulin resistance as a proinflammatory state: mechanisms, mediators, and therapeutic interventions.

Curr Drug Targets — Mayerson AB, Hundal RS, Dufour S et al The effects of rosiglitazone on insulin sensitivity, lipolysis, and hepatic and skeletal muscle triglyceride content in patients with type 2 diabetes.

Ruderman NB, Cacicedo JM, Itani S et al Malonyl—CoA and AMP-activated protein kinase AMPK : possible links between insulin resistance in muscle and early endothelial cell damage in diabetes. Biochem Soc Trans — Musi N, Hirshman MF, Nygren J et al Metformin increases AMP—activated protein kinase activity in skeletal muscle of subjects with type 2 diabetes.

Acta Physiol Scand — Al-Khalili L, Chibalin AV, Kannisto K et al Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci — Cline GW, Petersen KF, Krssak M et al Impaired glucose transport as a cause of decreased insulin-stimulated muscle glycogen synthesis in type 2 diabetes.

N Engl J Med — Park KS, Ciaraldi TP, Carter L et al Induction of insulin resistance in human skeletal muscle cells by downregulation of glycogen synthase protein expression.

Metabolism — Gaster M, Petersen I, Hojlund K, Poulsen P, Beck-Nielsen H The diabetic phenotype is conserved in myotubes established from diabetic subjects: evidence for primary defects in glucose transport and glycogen synthase activity. Gaster M, Schroder HD, Handberg A, Beck-Nielsen H The basal kinetic parameters of glycogen synthase in human myotube cultures are not affected by chronic high insulin exposure.

Biochim Biophys Acta — Henry RR, Ciaraldi TP, Mudaliar S, Abrams L, Nikoulina SE Acquired defects of glycogen synthase activity in cultured human skeletal muscle cells: influence of high glucose and insulin levels. Kausch C, Krutzfeldt J, Witke A et al Effects of troglitazone on cellular differentiation, insulin signaling, and glucose metabolism in cultured human skeletal muscle cells.

Wahl HG, Kausch C, Machicao F et al Troglitazone downregulates δ 6 desaturase gene expression in human skeletal muscle cell cultures. Park KS, Ciaraldi TP, Lindgren K et al Troglitazone effects on gene expression in human skeletal muscle of type II diabetes involve up-regulation of peroxisome proliferator-activated receptor-γ.

J Clin Endocrinol Metab — Park KS, Ciaraldi TP, Abrams-Carter L et al Troglitazone regulation of glucose metabolism in human skeletal muscle cultures from obese type II diabetic subjects.

Yonemitsu S, Nishimura H, Shintani M et al Troglitazone induces GLUT4 translocation in L6 myotubes. Seda O, Kazdova L, Krenova D, Kren V Rosiglitazone improves insulin resistance, lipid profile and promotes adiposity in a genetic model of metabolic syndrome X.

Folia Biol Praha — CAS Google Scholar. Musi N, Goodyear LJ AMP-activated protein kinase and muscle glucose uptake.

Jessen N, Pold R, Buhl ES et al Effects of AICAR and exercise on insulin-stimulated glucose uptake, signaling, and GLUT-4 content in rat muscles. J Appl Physiol — Abbud W, Habinowski S, Zhang J-Z et al Stimulation of AMP-activated protein kinase AMPK is associated with enhancement of GLUT1-mediated glucose transport.

Arch Biochem Biophys — Giannarelli R, Aragona M, Coppelli A, Del Prato S Reducing insulin resistance with metformin: the evidence today. Diabetes Metab S28—6S Download references. This study was supported by grants from the Swedish Research Council, the Thurings Foundation, the Swedish Medical Association, Tore Nilsons Stiftelse, the Novo-Nordisk Foundation, Harald and Greta Jeanssons Stiftelse, the Swedish Diabetes Association, the Markus and Amalia Wallenberg Foundation, the Swedish Fund for Research without Animal Experiments and the Swedish Central Board for Research Animal Welfare.

Department of Surgical Science, Karolinska Institute, Stockholm, Sweden. Goldstein, M. Article Google Scholar. Bergström, J. Google Scholar. Article ADS Google Scholar.

Hultman, E. to be published. Download references. Clinical Laboratory, St. Erik's Hospital, Stockholm. You can also search for this author in PubMed Google Scholar. Reprints and permissions.

BERGSTRÖM, J. Muscle Glycogen Synthesis after Exercise : an Enhancing Factor localized to the Muscle Cells in Man. Nature , — Download citation. Published : 01 April Issue Date : 16 April Anyone you share the following link with will be able to read this content:.

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European Journal of Applied Physiology Synthesis of muscle glycogen during recovery after prolonged severe exercise in diabetic and non-diabetic subjects.

Nesher R, Karl SE, Kipnis DM. Dissociation of effects of insulin and contraction on glucose transport in rat epitrochlearis muscle. Nilsson LH, Hultman E. Liver and muscle glycogen in man after glucose and fructose infusion. Scandinavian Journal of Clinical and Laboratory Investigation 5—10, Reed MJ, Brozinick JT, Lee MC, Ivy JL.

Muscle glycogen storage postexercise: effect of mode of carbohydrate administration. Richter EA, Garetto LP, Goodman NM, Ruderman NB. Muscle glycogen metabolism following exercise in the rat: increased sensitivity to insulin. Journal of Clinical Investigation —, Enhanced muscle glycogen metabolism after exercise: modulation by local factors.

Roach PJ, Lamer J. Rabbit skeletal muscle glycogen synthase. Enzyme phosphorylation state and effector concentrations as interacting control parameters. Roach PJ, Larner J. Covalent phosphorylation in the regulation of glycogen synthase activity.

Molecular and Cellular Biochemistry —, Roch-Norlund AE, Bergström J, Hultman E. Muscle glycogen and glycogen synthetase in normal subjects and in patients with diabetes mellitus: effect of intravenous glucose and insulin administration.

Scandinavian Journal of Clinical and Laboratory Investigation 77—84, Sherman WM. Carbohydrates, muscle glycogen and muscle glycogen supercompensation.

In Williams MH Ed. Ergogenic aids in sports, pp. Sherman WM, Costill DL, Fink WJ, Miller JM. The effect of exercise and diet manipulation on muscle glycogen and its subsequent utilization during performance.

International Journal of Sports Medicine 2: —, Sherman WM, Lamb DR. Nutrition and prolonged exercise. In Lamb DR Ed. Perspectives in exercise science and sports medicine: prolonged exercise, pp. Shreeve WW, Baker N, Miller M, et al. Metabolism 5: 22—29, Szanto S, Yudkin J.

The effect of dietary sucrose on blood lipids, serum insulin, platelet adhesiveness and body weight in human volunteers. Postgraduate Medical Journal —, Terjung RL, Baldwin KM, Winder WW, Holloszy JO.

Glycogen in different types of muscle and in liver after exhausting exercise. American Journal of Physiology —, Yakovlev NN. The effect of regular muscular activity on enzymes of glycogen, and glucosephosphate in muscles and liver. Biochemistry —, Young AA, Bogardus C, Stone K, Mott DM.

Insulin response of components of whole-body and muscle carbohydrate metabolism in humans. American Journal of Physiology ; E—E, Zakin D, Herfman RH, Gordon WC. The conversion of glucose and fructose to fatty acids in the human liver.

Biochemical Medicine 2: —, Download references. Exercise Physiology and Metabolism Laboratory, Department of Kinesiology, University of Texas at Austin, Austin, Texas, USA. You can also search for this author in PubMed Google Scholar. Reprints and permissions.

Ivy, J. Muscle Glycogen Synthesis Before and After Exercise. Sports Med 11 , 6—19 Download citation. Published : 09 October Issue Date : January Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative. Summary The importance of carbohydrates as a fuel source during endurance exercise has been known for 60 years.

Access this article Log in via an institution. References Adolfsson S. Acta Physiologica Scandinavica —, Article PubMed CAS Google Scholar Ahlborg BG, Bergström J, Brohult J, Ekelund LG, Hultman E, et al. Foersvarsmedicin 3: 85—99, a Google Scholar Ahlborg B, Bergström J, Ekelund LG, Hultman E.

Acta Physiologica Scandinavica —, b Article CAS Google Scholar Bergström J, Hermansen L, Hultman E, Saltin B. Acta Physiologica Scandinavica —, Article PubMed Google Scholar Bergström J, Hultman E.

Scandinavian Journal of Clinical and Laboratory Investigation —, a Article PubMed Google Scholar Bergström J, Hultman E. Nature —, b Article Google Scholar Bergström J, Hultman E.

Acta Medica Scandinavica 93—, c Article PubMed Google Scholar Bergström J, Hultman E, Roch-Norlund AE. Scandinavian Journal of Clinical and Laboratory Investigation —, Article PubMed Google Scholar Blom PCS, Høstmark AT, Vaage O, Kardel KR, Maehlum S. Medicine and Science in Sports and Exercise —, PubMed CAS Google Scholar Christensen EH, Hansen O.

Scandinavian Archives of Physiology —, a Article Google Scholar Christensen EH, Hansen O. Scandinavian Archives of Physiology —, b Article Google Scholar Costill DL, Bowers R, Branam G, Sparks K. Journal of Applied Physiology —, PubMed CAS Google Scholar Costill DL, Sherman WM, Fink WJ, Maresh C, Witten M, et al.

American Journal of Clinical Nutrition —, PubMed CAS Google Scholar Danforth WH. Journal of Biological Chemistry —, PubMed CAS Google Scholar Fell RD, Terblanche SE, Ivy JL, Young JC, Holloszy JO.

Journal of Applied Physiology —, PubMed CAS Google Scholar Fischer EH, Heilmeyer LMG, Haschke RH. Current Topics in Cellular Regulation 4: —, CAS Google Scholar Grollman S.

Journal of Experimental Zoology —, Article CAS Google Scholar Guinovart JJ, Salavert A, Massague J, Ciudad CJ, Salsas E, Itarte E. FEBS Letters —, Article PubMed CAS Google Scholar Hermansen L, Hultman E, Saltin B. Acta Physiologica Scandinavica —, Google Scholar Hodges RE, Krehl WA.

American Journal of Clinical Nutrition —, PubMed CAS Google Scholar Holloszy JO, Narahara HT.

The aim of Enhancinh study was Glycemic index and blood sugar impact determine the effect of several antidiabetic agents synthesiz insulin-stimulated glycogen synthesis, flycogen well as on mRNA Fueling for strength training. After this, insulin-stimulated snythesis synthesis was Fueling for strength training. mRNA levels of the Well-maintained fat distribution transporters GLUT1 and GLUT4, the peroxisomal proliferator activator receptor gamma PPAR gamma co-activator 1 PGC1 and the myocyte-specific enhancer factors MEF2MEF2A, MEF2C and MEF2D were determined using real-time PCR analysis after 8 days exposure to the various antidiabetic agents. Insulin-stimulated glycogen synthesis was significantly increased in cultured human myotubes treated with insulin, rosiglitazone or metformin for 8 days, compared with non-treated cells. In contrast, treatment with AICAR was without effect on insulin-mediated glycogen synthesis. BMC Cell Biology volume EnhnacingArticle glycogwn 9 Glyycogen this article. Clean eating snacks details. Branched-chain amino acids, especially leucine, are known Ehnancing interact with insulin signaling Enhancing glycogen synthesis and Enhancing glycogen synthesis metabolism. However, the mechanism Nutritional science which this is exerted, remain to be clearly defined. In order to examine the effect of leucine on muscle insulin signaling, a set of experiments was carried out to quantitate phosphorylation events along the insulin signaling pathway in human skeletal muscle cell cultures. Leucine is shown to increase the magnitude of insulin-dependent phosphorylation of protein kinase B AKT at Ser and glycogen synthase kinase GSK3β at Ser