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Agar well diffusion assayAntifungal activity assessment -
Controls articles are prepared in the same fashion and treated under the same parameters as the test articles. Test and control articles are wetted with a non-ionic wetting agent to aid in the absorption of the inoculum.
Mineral Salts Agar plates are prepared per each test and control article, and each plate is evenly inoculated with 0. Once test and control articles have been placed on their respective agar plates, the test and control articles are inoculated with 0.
The specimens are incubated at 30 o C ± 2 o C for 14 days. Upon completion of the incubation period, photographs are taken, and each article is qualitatively assessed for growth. Growth is evaluated macroscopically with the unaided eye, and microscopically, with a 10X microscope, and growth ratings are assigned.
Strengths of the AATCC 30 The AATCC 30 test is a test that evaluates the antifungal capabilities of the test substance under fairly stringent conditions.
This makes it a good indicator of antifungal efficacy, compared to other, relatively less stringent methods. The test substance comes into contact with a high number of spores for increased efficacy. A more definitive test than many other fungal methods, any inhibition of growth shown in this test is due to the antimicrobial effect of the test substance rather than the inability of the fungus to find nutrients and grow.
Quicker incubation period than other fungal test methods 14 days vs. This test method caters to fabrics with the incorporation of the non-ionic wetting agent. Inoculum contacts both faces of the material, allowing for full absorption into the fabric. Weaknesses of the AATCC 30 The stringency of the test might overwhelm milder antifungal agents.
Inherent variability in spore concentration can lead to increased variability when running the test, as the AATCC 30 does not require the spore suspension to be standardized.
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Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IBSAM, ESIAB, Université de Brest, EA, Technopôle Brest — Iroise, , Plouzané, France.
You can also search for this author in PubMed Google Scholar. Correspondence to Jérôme Mounier. Reprints and permissions. Lačanin, I.
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